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BRUENING, George E. Professor of Plant Pathology Department of Plant
Pathology |
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| Degree | Institution | Year | Specialization | |||
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| B.S. | Carroll College, Waukesha, Wisconsin | 1960 | Chemistry | |||
| M.S. | University of Wisconsin, Madison | 1963 | Biochemistry | |||
| Ph.D. | University of Wisconsin, Madison | 1965 | Biochemistry | |||
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| Area of Specialization | ||||||
| Mechanisms of natural and genetically engineered resistance against viruses; biochemistry and molecular genetics of plant viruses and subviral RNA agents | ||||||
| Research Statement | ||||||
| Plant genes for resistance against pathogens usually provide the most economical and environmentally sound approach to controlling diseases in crop agriculture. We seek to understand what are the signals from the pathogen, particularly a virus, that the resistant plant recognizes. How does the plant respond to interfere with pathogen accumulation? Genetically engineered resistance also is under study, using nucleotide sequences derived from virus and factors selected for their ability to bind to and inhibit proteases and other virus enzymes. Satellite RNAs and certain other dependent RNAs act as molecular parasites, of the associated plant viruses, without gene expression. The structure, function, and virus associations of satellite RNAs are investigated to understand satellite RNA replication and control over virus replication and symptom induction. Additionally, we are investigating the mechanisms of RNA-catalyzed reactions and their uses as ribozymes. The described research problems require molecular biology and biochemistry for their solutions, and a variety of in vitro and in vivo methods are in use. | ||||||
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Selected
Publications
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| Transgenes Are Revolutionizing Crop Production. Bruening, G. 2000. California Agriculture 54(4):36-46. | ||||||
| Evidence for Participation of RNA 1-Encoded Elicitor in Cowpea Mosaic Virus-Mediated Concurrent Protection. Bruening, G., Buzayan, J. M., Ferriero, C., and Lim, W. 2000. Virology 266: 299-309. | ||||||
| Plant Gene Silencing Regularized. G. Bruening. 1998. Proc. Nat. Acad. Sci. 95: 13349-13351. | ||||||
| Formation of Circular Satellite Tobacco Ringspot Virus RNA in Protoplasts Transiently Expressing the Linear RNA. Chay, C. A., X. Guan, and G. Bruening. 1997. Virology 239:413-425. | ||||||
| Chemical Cleavage of 5'-Linked Protein from Tobacco Ringspot Virus Genomic RNAs and Characterization of the Protein-RNA Linkage. Zalloua, P. A., J. M. Buzayan and G. Bruening. 1996. Virology 219:1-8. | ||||||
| Effective Ribozyme Delivery in Plant Cells. Perriman, R., G. Bruening, E. S. Dennis, and W.J. Peacock. 1995. Proc. Nat. Acad. Sci. USA 92:6175-6179. | ||||||
| Increase in Satellite Tobacco Ringspot Virus RNA Initiated by Inoculating Circular RNA. Buzayan, J. M., H. van Tol, P. A. Zalloua, and G. Bruening. 1995. Virology 208:832-837. | ||||||
| Transient Gene Expression of Antisense RNA and Coat Protein-Encoding Sequences Reduced Accumulation of Cherry Leafroll Virus in Tobacco Protoplasts. Brooks, M., and G. Bruening. 1995. Virology 208:132-141. | ||||||